Spore Suspensions are diluted aliquots from a primary spore crop. Used to directly inoculate (and challenge) a material or solution with a known concentration of spores, Mesa’s Spore Suspensions are offered in either 100% deionized water or in a 40% ethanol solution. To determine which configuration should be used, the materials being inoculated should be evaluated.
Users who are inoculating some type of carrier material may prefer to use an ethanol suspension of spores due to the faster drying time that the ethanol provides. In addition, some hydrophobic materials may cause a water droplet (0.1ml) to bead on the surface. As this bead dries, the spores within that droplet may form spore aggregates with the spores piling on top of one another. For these types of materials it would be an advantage to use an ethanol solution so the spores spread more evenly over the carrier surface.
Those who are inoculating some type of pharmaceutical product may prefer to use an aqueous suspension of spores as the water may not interact with the chemical components within the product as the ethanol would. Conversely, an aqueous solution may not be miscible in a solution containing an oil-based component. Utilizing an ethanol suspension of spores would allow the spores to be evenly dispersed throughout the solution giving the most accurate and relevant results.
To learn more about DI water and Ethanol solution spore suspensions, contact Mesa Labs.
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