Culture Media for Biological Indicators

“The manufacturer should provide directions for use, including the medium and conditions to be used for the recovery of micro-organisms after exposure to the sterilization process.”   – USP <1229.5>

• Soybean Casein Digest Broth is the recommended media for use with Biological Indicators. Use of another media may detrimentally affect outgrowth of spores.
• Mesa uses Difco media in-house for the manufacture and QC testing of our products. Mesa recommends Difco TSA and TSB for use in verifying the certified label claims for our products

Tryptic Soy Broth with Bromocresol Purple
TSB-BP16

• Mesa’s TSB with Bromocresol purple pH indicator is specially formulated for use with our Spore Strip biological indicators
• A reduced incubation time of 24 hours at 60 °C +/- 2 °C has been validated for steam sterilization when the Mesa Bacterial Spore Strip (either the single species G. stearothermophilus spore strip or the dual species spore strip) is used in conjunction with Mesa’s Modified Tryptic Soy Broth with Bromocresol Purple (TSB-BP16).
• Prepared Media is packaged in 16mm x 100mm Type I borosilicate glass tubes with polypropylene screwcaps (white rubber liners).

Releasat^®

Releasat media is specially formulated soybean casein digest culture medium containing a pH color indicator which turns a dramatic yellow when spores grow.

• Releasat medium is used to culture the MesaStrip or Apex discs in place of “generic” soybean casein digest medium.
• Releasat Red media is specially formulated for rapid outgrowth of B. atrophaeus spores that may have survived the Ethylene Oxide or Dry Heat process.
• Releasat Purple media is specially formulated for rapid outgrowth of G. stearothermophilus spores that may have survived steam and hydrogen peroxide sterilization/decontamination processes.
• Prepared Media is packaged in 16mm x 100mm Type I borosilicate glass tubes with polypropylene screwcaps (white rubber liners).
• Releasat culture kits are available for monitoring ethylene oxide, dry heat and chlorine dioxide sterilization processes.

Population Assay Materials

Our Population Assay Materials contain everything required to perform accurate population assays of Mesa Labs’ biological indicator products. 

Detailed population assay instructions are on the individual product pages.

PAK-M* (with Difco media)

• • • Growth medium only (240ml)

 

 

PAK-G*

• Glassware and pipettes only

 

* Use both product codes for complete population assay materials